水稻千粒重QTL qTGW1.1的验证

doi:10.3969/j.issn.1001-7216.2014.02.003

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 125-131.DOI: 10.3969/j.issn.1001-7216.2014.02.003

水稻千粒重QTL qTGW1.1的验证

张宏伟,朱玉君,陈玉宇,陈俊宇,黄得润,庄杰云^*

中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室, 杭州 310006；

收稿日期:2013-11-18 修回日期:2013-12-21 出版日期:2014-03-10 发布日期:2014-03-10
通讯作者: 庄杰云*
基金资助:
国家863计划资助项目（2012AA101102）；国家超级稻育种专项（201301）；中央级公益性科研院所基本业务费专项（2012RG0023)

Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)

ZHANG Hongwei, ZHU Yujun, CHEN Yuyu, CHEN Junyu, HUANG Derun, ZHUANG Jieyun^*

Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;

Received:2013-11-18 Revised:2013-12-21 Online:2014-03-10 Published:2014-03-10
Contact: ZHUANG Jieyun*

摘要/Abstract

摘要： 对前期定位在水稻第1染色体长臂上3.7 Mb区间内的千粒重QTL qTGW1.1进行验证，并提高其定位精度。从珍汕973/密阳46 BC2F5群体中筛选到1个在RM11448-RM11615区间杂合的单株，应用SSR标记检测其衍生的BC2F6和BC2F7群体，筛选得到杂合区间分别为RM11448-RM11522、RM11448-RM11549和RM1232-RM11615的BC2F7单株各1个。种植3个BC2F8群体，筛选在整个分离区间呈单一亲本型的单株，自交后获得在对应区间呈两种基因型的近等基因系各1套，大田种植，考查抽穗期、千粒重、粒长和粒宽，应用SAS软件对同一群体中不同基因型间的表型差异进行方差分析。在千粒重和粒形性状上，异质区间为RM11448-RM11522的近等基因系未呈显著变异，而另2套材料检测到显著的基因型作用，且其增效等位基因均来自珍汕97；对抽穗期，3套材料均未检测到显著作用。通过比较3个异质区间的基因组位置，将qTGW1.1界定于RM11522和RM11554之间688.8 kb的区间内。

关键词: 水稻（Oryza sativa L.）, 数量性状座位, 微效作用, 千粒重, 验证

Abstract: This study was conducted to validate a quantitative trait locus (QTL) for 1000grain weight (TGW) which was previously mapped in a 3.7 Mb region on the long arm of rice chromosome 1, and to locate this QTL more precisely. A plant carrying a heterozygous segment extending from RM11448 to RM11615 was selected from the Zhenshan 973/Milyang 46 BC2F5 population. The resulting BC2F6 and BC2F7 populations were assayed with SSR markers. Three BC2F7 plants were identified, carrying heterozygous segments covering the intervals RM11448-RM11522, RM11448-RM11549 and RM1232-RM11615, respectively. Nonrecombinant homozygotes were identified from the BC2F8 populations, from which three sets of near isogenic lines (NILs) in the BC2F8∶9 generation were produced, consisting of two parental genotypes in the intervals RM11448-RM11522, RM11448-RM11549 and RM1232-RM11615, respectively. Field trials were conducted to measure heading date (HD), TGW, and grain shape traits including grain length and width. Twoway analysis of variance was performed to test the phenotypic differences between two genotypic groups in each NIL set using SAS program. No significant variations were observed on TGW and grain shape in the NIL which was heterogeneous in the interval RM11448-RM11522, whereas significant genotypic effects were detected in the remaining two NILs in which the enhancing alleles were all derived from Zhenshan 97. For HD, no significant effects were detected in the three NILs. Based on comparison among genomic locations of the three heterogeneous regions, qTGW1.1 was delimited to a 688.8 kb region flanked by RM11522 and RM11554.

Key words: rice (Oryza sativa L.), quantitative trait locus, minor effect, 1000grain weight, validation

中图分类号:

张宏伟,朱玉君,陈玉宇,陈俊宇,黄得润,庄杰云*. 水稻千粒重QTL qTGW1.1的验证[J]. 中国水稻科学, 2014, 28(2): 125-131.

ZHANG Hongwei, ZHU Yujun, CHEN Yuyu, CHEN Junyu, HUANG Derun, ZHUANG Jieyun*. Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)[J]. Chinese Journal of Rice Science, 2014, 28(2): 125-131.

参考文献

\[1\]Song X J， Huang W， Shi M， et al. A QTL for rice grain width and weight encodes a previously unknown RINGtype E3 ubiquitin ligase. Nat Genet, 2007， 39： 623630.

\[2\]Fan C C， Xing Y Z， Mao H L， et al. GS3，a major QTL for grain length and weight and minor QTL for grain width and thickness in rice，encodes a putative transmembrane protein. Theor Appl Genet, 2006， 112： 11641171.

\[3\]Qi P， Lin Y S， Song X J， et al. The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating CyclinT1;3. Cell Res, 2012， 22： 16661680.

\[4\]Zhang X J， Wang J F， Huang J， et al. Rare allele of OsPPKL1 associated with grain length causes extralarge grain and a significant yield increase in rice. Proc Natl Acad Sci USA, 2012，109： 2153421539.

\[5\]Li Y B，Fan C C，Xing Y Z，et al. Natural variation in GS5 plays an important role in regulating grain size and yield in rice. Nat Genet, 2011， 43： 12661269.

\[6\]Shomura A， Izawa T， Ebana K， et al. Deletion in a gene associated with grain size increased yields during rice domestication. Nat Genet, 2008， 40： 10231028.

\[7\]Weng J F， Gu S H， Wan X Y， et al. Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight. Cell Res, 2008， 18： 11991209.

\[8\]Ishimaru K， Hirotsu N， Madoka Y， et al. Loss of function of the IAAglucose hydrolase gene TGW6 enhances rice grain weight and increases yield. Nat Genet, 2013， 45： 707711.

\[9\]Wang S K， Wu K， Yuan Q B， et al. Control of grain size, shape and quality by OsSPL16 in rice. Nat Genet, 2012， 44： 950954.

\[10\]Yu S B， Li J X， Xu C G， et al. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc Natl Acad Sci USA, 1997， 94： 92269231.

\[11\]Li J X，Yu S B，Xu C G，et al. Analyzing quantitative trait loci for yield using a vegetatively replicated F2 population from a cross between the parents of an elite rice hybrid. Theor Appl Genet, 2000， 101： 248254.

\[12\]Xing Y Z， Tan Y F， Hua J P， et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Theor Appl Genet, 2002， 105： 248257.

\[13\]Hua J P， Xing Y Z， Xu C G， et al. Genetic dissection of an elite rice hybrid revealed that heterozygotes are not always advantageous for performance. Genetics, 2002， 162： 18851895.

\[14\]余守武，杨长登，樊叶杨，等. 水稻第1染色体千粒重QTL的遗传分解. 科学通报， 2008， 53(12)： 13891394.

\[15\]Shao G N， Wei X J， Chen M L， et al. Allelic variation for a candidate gene for GS7, responsible for grain shape in rice. Theor Appl Genet, 2012， 125： 13031312.

\[16\]Xie X B， Jin F X， Song M H， et al. Fine mapping of a yieldenhancing QTL cluster associated with transgressive variation in an Oryza sativa × O. rufipogon cross. Theor Appl Genet, 2008， 116： 613622.

\[17\]冯建成. 水稻区试品种产量、农艺性状及育种分析. 福建稻麦科技， 2010， 28(2)： 912.

\[18\]刘传光，周汉钦，冯道基，等. 影响华南稻区常规籼稻产量水平的主要农艺性状分析. 中国水稻科学， 2012， 26(2)： 182188.

\[19\]Guo L， Wang K， Chen J Y， et al. Dissection of two quantitative trait loci for grain weight linked in repulsion on the long arm of chromosome 1 of rice (Oryza sativa L.). Crop J, 2013， 1(1)：7076.

\[20\]施勇烽，应杰政，王磊，等. 鉴定水稻品种的微卫星标记筛选. 中国水稻科学， 2005， 19(3)： 195201.

\[21\]Chen X， Temnykh S， Xu Y， et al. Development of a microsatellite framework map providing genomewide coverage in rice (Oryza sativa L.). Theor Appl Genet, 1997， 95： 553567.

\[22\]SAS Institute Inc. SAS/ STAT User’s Guide. Cary， NC: SAS Institute， 1999.

\[23\]Dai W M， Zhang K Q， Wu J R， et al. Validating a segment on the short arm of chromosome 6 responsible for genetic variation in the hull silicon content and yield traits of rice. Euphytica, 2008， 160： 317324.

\[24\]龚金龙，胡雅杰，龙厚元，等. 大穗型杂交粳稻产量构成因素协同特征及穗部性状. 中国农业科学， 2012， 45(11)： 21472158.

\[25\]郭梁，张振华，庄杰云. 水稻抽穗期QTL及其与产量性状遗传控制的关系. 中国水稻科学， 2012， 26(2)： 235245.

\[26\]Zhuang J Y， Fan Y Y， Rao Z M， et al. Analysis on additive effects and additivebyadditive epistatic effects of QTLs for yield traits in a recombinant inbred line population of rice. Theor Appl Genet, 2002， 105： 11371145.

\[27\]Bai X， Wu B，Xing Y. Yieldrelated QTLs and their applications in rice genetic improvement. J Integr Plant Biol, 2012，54(5)： 300311.

[1]	闫浩亮, 王松, 王雪艳, 党程成, 周梦, 郝蓉蓉, 田小海. 不同水稻品种在高温逼熟下的表现及其与气象因子的关系[J]. 中国水稻科学, 2021, 35(6): 617-628.
[2]	李盼盼, 朱玉君, 郭梁, 庄杰云, 樊叶杨. 利用剩余杂合体衍生的近等基因系精细定位水稻粒长微效QTL qGL1.1[J]. 中国水稻科学, 2020, 34(2): 125-134.
[3]	孟峰, 张亚玲, 靳学慧. 黑龙江省稻瘟病菌无毒基因AVR-Pita及其同源基因的检测与分析[J]. 中国水稻科学, 2020, 34(2): 143-149.
[4]	朱安东, 孙志超, 朱玉君, 张荟, 牛小军, 樊叶杨, 张振华, 庄杰云. 应用剩余杂合体衍生群体定位水稻粒重粒形QTL[J]. 中国水稻科学, 2019, 33(2): 144-151.
[5]	杨雪芹, 陈婷婷, 赵霞, 张彩霞, 杨永杰, 符冠富, 陶龙兴. 赤霉素与多效唑协同影响超级稻甬优12产量形成的作用途径分析[J]. 中国水稻科学, 2016, 30(1): 53-61.
[6]	王琳琳, 陈玉宇, 郭梁, 张宏伟, 樊叶杨, 庄杰云. 水稻第1染色体qTGW1.2区域粒重组分性状QTL的剖析[J]. 中国水稻科学, 2015, 29(3): 232-240.
[7]	郭旻,李荣德,姚健,朱娟,范祥云,王伟,汤述翥,顾铭洪,严长杰*. 水稻叶片形态相关基因RL3(t)的遗传分析和精细定位[J]. 中国水稻科学, 2014, 28(5): 458-464.
[8]	王军1 ,杨杰1 ,徐祥2 ,朱金燕1 ,范方军1 ,李文奇1 ,王芳权1 ,仲维功1,*. 水稻千粒重基因TGW6功能标记的开发与利用[J]. 中国水稻科学, 2014, 28(5): 473-478.
[9]	陈俊宇,王凯,龚俊义,樊叶杨,黄得润,庄杰云*. RFT1与Hd1所在区间对水稻抽穗期、株高和千粒重的作用[J]. 中国水稻科学, 2013, 27(2): 117-121.
[10]	罗炬#,邵高能#,魏祥进,陈明亮,唐绍清,焦桂爱,谢黎虹,胡培松* . 一个控制水稻株高QTL qPH3的遗传分析[J]. 中国水稻科学, 2012, 26(4): 417-422.
[11]	郭媛, 程保山洪德林,. 粳稻SSR连锁图谱的构建及恢复系卷叶性状QTL分析[J]. 中国水稻科学, 2009, 23(3): 245-251 .
[12]	沈波, 蒋靓, 於卫东, 樊叶杨, 庄杰云,. 水稻苗期盐胁迫下叶绿素荧光参数的QTL分析 [J]. 中国水稻科学, 2009, 23(3): 319-322 .
[13]	程式华,曹立勇,庄杰云,吴伟明. 关于超级稻品种培育的资源和基因利用问题[J]. 中国水稻科学, 2009, 23(3): 223-228 .
[14]	邵高能.唐绍清.焦桂爱.罗炬.唐傲,胡培松. 稻米蒸煮品质性状的QTL定位 [J]. 中国水稻科学, 2009, 23(1): 94-94～98 .
[15]	王辉,曹立勇,郭玉华,程式华,. 水稻生理特性与抗旱性的相关分析及QTL定位[J]. 中国水稻科学, 2008, 22(5): 477-484 .

水稻千粒重QTL qTGW1.1的验证

Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics